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Sharka (Plum pox virus) of stone fruits
JAKUBÍKOVÁ, Hedvika
Plum pox virus causes one of the most devastating stone fruit diseases. The main target of this thesis is a detection of Plum pox virus in infected leaf tissue of Prunus domestica.. The presence of the virus in a plant material was confirmed by the RNA isolation and its use as a template for RT-PCR. Furthermore, the basic information about properties of the virus, common methods of the virus detection and protection possibilities against the virus is are included.
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Metody eliminace virů u meruněk a broskvoní
Fronková, Hana
This work describes methods of virus elimimination for obtaining virus-free plant material of peaches and apricots. Four methods of elimination were chosen, apical meristem isolation, thermotherapy, chemotherapy and cryotherapy. In this work, four economical singificant viruses are described, which can infect peaches and apricots. This work describes botanical characteristic above-mentioned fruit species and importance of their planting in the area of the Czech Republic.
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Methodology for the phenotyping resistance to Plum pox virus in peach
Salava, Jaroslav ; Polák, Jaroslav
Sharka disease, which is caused by the Plum pox virus (PPV), is the most economically important virus disease in fruit trees worldwide. Since it is not possible to completely eradicate PPV in the Czech Republic, it is necessary to find another way to control this pathogen. The most promising seems to be the breeding of new cultivars for resistance. This methodology provides a comprehensive protocol for testing PPV resistance in peach. It is based on visual assessment of disease symptoms on leaves and fruits, and determining the presence of the virus in plants using ELISA and RT-PCR techniques.
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Detection of selected plant viruses by microarrays
HRABÁKOVÁ, Lenka
The main aim of this master thesis was the simultaneous detection of four selected plant viruses ? Apple mosaic virus, Plum pox virus, Prunus necrotic ringspot virus and Prune harf virus, by microarrays. The intermediate step in the process of the detection was optimizing of multiplex polymerase chain reaction (PCR).
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